Introduction to Isoform Analysis
Module 5 - Key concepts
- alternative expression, splicing, differential splicing analysis
Module 5 - Learning objectives
- Use StringTie in modes that facilitate transcript/isoform discovery
- Use Cuffmerge to combine transcriptomes from multiple StringTie runs and compare assembled transcripts to known transcripts
- Learn how to perform differential splicing analysis with Ballgown
- Examine junctions counts with RegTools and StringTie alternative transcript files at the command line
- Visualize junction counts and StringTie assembled transcripts in IGV
Lecture
